Research

Submarine canyons are ubiquitous along continental margins, and often thought of as key conduits for the transfer of terrestrial sediment, nutrients and organic material to the deep sea. During sea level highstands, most of this shelf to deep-sea transport occurs on an episodic basis, driven by seabed resuspension in and around the canyon head, associated with large storms, floods, earthquakes and other energetic events that are thought to induce gravity-driven, downslope sediment flows. However, the processes that control canyon flushing and sediment infilling remain poorly understood. To date, most of the direct monitoring of these processes has been focused on large submarine canyon and fjord systems that are highly active during the current sea level highstand. This proposed effort is a collaborative, interdisciplinary effort with the goal of understanding the origin of modern deep-water stratigraphy associated with submarine canyon dynamics. The observational component will deploy two instrumented arrays in canyons of differing morphology (Astoria Canyon and Quinault Canyon) to develop a spatially distributed minute-scale record of water and particulate transport processes over a complete annual cycle. This data would yield the timing and magnitude of canyon transport processes, and help constrain the sedimentary response to oceanographic disturbances. A seasonal seabed surface core survey in the region will examine annual depositional cycles, and allow for the direct testing of seafloor remobilization thresholds along canyon thalwegs. All of these data will then be used as model inputs, providing the constraints for locally-parameterized hydrodynamic modeling of sediment gravity-flow triggering and runout distances.

Investigating the impact of metallic structures on electromagnetic waves near the ocean surface.

In January/February 2024, WHOI will be installing new multibeam and subbottom profiler systems to the RV Neil Armstrong. These systems include upgrading to Kongsberg EM124 and EM712 multibeams, and installing a new array for a Kongsberg SBP29. Part of the installation will also include adding a new Seapath MRU to replace the existing POSMV system as the primary MRU. A requirement of major transducer installation and improvements, are Sea Acceptance Tests (SAT) and scientifically focused system calibration. Often a manufacturer SAT is not robust enough to ensure highest scientific returns, hence why more in depth calibrations are required. It is also critical that calibrations are conducted at predetermined locations, as advised by the UNOLS Multibeam Advisory Committee (MAC) and the SBP29 specialist contractor, Dan Orange. Planning for a mid-Atlantic shipyard, the closest available site for conducting proper calibration of the systems is at the slope on "Blake Nose", about 25 hr steam, in good weather.

Our study will use cutting-edge in situ imaging (using a UVP-DEEP) of Rhizaria to bypass limitations in other sampling methods and to produce accurate estimates of Rhizaria biomass. Additionally, sampling will occur across a wide depth range (0-1200 m), at monthly intervals, and in conjunction with a wide array of other biological, chemical, and physical oceanographic measurements (as part of the Bermuda Atlantic Time-series Study, BATS). UVP imagery data will be integrated with molecular identification of Rhizaria using community metabarcoding and barcoding of individual Rhizaria collected from MOCNESS net tows taken in conjunction with UVP casts. Size-specific elemental analyses of Rhizaria and seasonally and vertically explicit estimates of Rhizaria biomass will be used to model their contributions to vertical flux in the system.

Long-term observations over several decades are a powerful tool for investigating ocean physics, biology, and chemistry, and the response of the oceans to environmental change. The Bermuda Atlantic Time-Series Study, known as BATS, has been running continuously since 1988. The research goals of the BATS program are: (1) to improve our understanding of the time-varying components of the ocean carbon cycle and the cycles of related nutrient elements such as nitrogen, phosphorus, and silicon; and, (2) to identify the relevant physical, chemical and ecosystem properties responsible for this variability. In addition, the BATS program has strong and diverse broader impacts, contributing to the field of ocean sciences by providing high quality ocean observations and data for seagoing scientists and modelers, and a framework through which researchers can conceive and test hypotheses. This award will support the operations of the BATS program for five more years. The primary BATS research themes are as follows: (1) Quantify the role of ocean-atmosphere coupling and climate variability on air-sea exchange of CO2, and carbon export to the ocean interior; (2) Document trends and the controls on the interannual to decadal scale variability in carbon and nutrient cycles to their coupling in the surface and deep ocean via the Redfield Ratio paradigm; (3) Quantify the response of planktonic community structure and function, and impact on biogeochemical cycles to variability in surface fluxes and dynamical processes; (4) Facilitate development, calibration and validation of next generation oceanographic sensors, tools and technologies; and, (5) Generate a dataset that can be utilized by empiricists, modelers and students. This research integrates ocean physics, chemistry and biology into a framework for understanding oceanic processes and ocean change in the North Atlantic subtropical gyre. The existing 29 years of BATS data provide robust constraints on seasonal and interannual variability, the response of the Sargasso Sea ecosystem to natural climate variability, and signal detection of potential ocean changes. This project would extend the BATS program through years 31-35 to address a series of ten interlinked questions through integrated research approaches and a multitude of collaborative efforts. In addition to the themes above, and embedded into the ten questions and approaches, the BATS team will focus on, for example, coupling of particle production and biogeochemistry; revisiting the complexities of the biological carbon pump; oxygen decline; and changes in the hydrography, physics, ocean carbon cycle and biogeochemistry of the Sargasso Sea. The highest quality data observation and collection will be maintained and used to address these questions. Importantly, a wide range of collaborations at the BATS site, spanning the physical and biogeochemical disciplines, will aid these broad goals. Strong links to community stakeholders, and close collaboration (including methods intercomparisons and personnel exchanges) with the Hawaii Ocean Time-series are proposed. This work will extend the research findings of the project into educational and training opportunities within and beyond the oceanographic community, including training and mentorship of both undergraduate and graduate students.

One of the longest open-ocean hydrographic stations in the world is maintained at the Hydrostation S site (formerly known as the Panulirus site) located ~25 km southeast of Bermuda in the North Atlantic Ocean. This program of repeat biweekly hydrographic observations began in 1954 (by Hank Stommel) and now, in its seventh decade, has proved to be the catalyst for numerous studies of ocean physics, biological processes and biogeochemistry. Hydrostation S continues to be recognized as one of the most important sustained ocean time–series. The renewal of the Hydrostation S program is proposed herein, extending this invaluable time–series of ocean hydrography to almost 70 years. The upper ocean warming trend has strengthened (about +1.4° C since the 1970's; ~+0.8°C in last decade) with significant salinification (+0.16). Deeper waters such as the Labrador Sea Water has cooled by a few tenths of a degree and freshened with a marked decadal oscillation. The signature of deoxygenation has been observed at Hydrostation S in the upper ocean (about 4 µmoles kg -1 decade -1 decrease in dissolved oxygen) as well as the oxygen minimum zone (~2.5 µmoles kg -1 decade -1 ). Hydrostation S also supports the longest global ocean CO 2 and acidification time-series (from 1983 to present) The major objective of the proposal is to continue Hydrostation S into the eighth decade with numerous questions related to warming and cooling, salinification and freshening, deoxygenation and insights on biogeochemical changes over time. This program constitutes frequent water column sampling of temperature, salinity, and dissolved oxygen (and indirectly, sampling of important ocean carbon time–series) of the North Atlantic subtropical gyre at the Hydrostation S site. Such work is complementary to other sustained observations such as the Bermuda Atlantic Time–series Study (BATS) and Ocean Flux Program (OFP). We propose to carry out a similar sampling format that has been followed for the past 68 years. As for the past ten years, we would continue to conduct two CTD profiles to better capture the deep–water variability while maintaining all the previous discrete depths. The first CTD cast will profile to full ocean depth (3,300–3,500 m) while the second CTD cast will profile to 500 m (to allow for instrumentation not rated for full ocean depth such as a PAR sensor). One indirect objective will be to continue to collaboratively evaluate a BIOS-based glider program with Hydrostation S data as critically important ground truthing data. The existing glider program has challenging issue, and the aim of this effort is to use robust and highly accurate Hydrostation S data to test the capability of emerging technologies over the next five to ten years.

One of the longest open-ocean hydrographic stations in the world is maintained at the Hydrostation S site (formerly known as the Panulirus site) located ~25 km southeast of Bermuda in the North Atlantic Ocean. This program of repeat biweekly hydrographic observations began in 1954 (by Hank Stommel) and now, in its seventh decade, has proved to be the catalyst for numerous studies of ocean physics, biological processes and biogeochemistry. Hydrostation S continues to be recognized as one of the most important sustained ocean time–series. The renewal of the Hydrostation S program is proposed herein, extending this invaluable time–series of ocean hydrography to almost 70 years. The upper ocean warming trend has strengthened (about +1.4° C since the 1970's; ~+0.8°C in last decade) with significant salinification (+0.16). Deeper waters such as the Labrador Sea Water has cooled by a few tenths of a degree and freshened with a marked decadal oscillation. The signature of deoxygenation has been observed at Hydrostation S in the upper ocean (about 4 µmoles kg -1 decade -1 decrease in dissolved oxygen) as well as the oxygen minimum zone (~2.5 µmoles kg -1 decade -1 ). Hydrostation S also supports the longest global ocean CO 2 and acidification time-series (from 1983 to present) The major objective of the proposal is to continue Hydrostation S into the eighth decade with numerous questions related to warming and cooling, salinification and freshening, deoxygenation and insights on biogeochemical changes over time. This program constitutes frequent water column sampling of temperature, salinity, and dissolved oxygen (and indirectly, sampling of important ocean carbon time–series) of the North Atlantic subtropical gyre at the Hydrostation S site. Such work is complementary to other sustained observations such as the Bermuda Atlantic Time–series Study (BATS) and Ocean Flux Program (OFP). We propose to carry out a similar sampling format that has been followed for the past 68 years. As for the past ten years, we would continue to conduct two CTD profiles to better capture the deep–water variability while maintaining all the previous discrete depths. The first CTD cast will profile to full ocean depth (3,300–3,500 m) while the second CTD cast will profile to 500 m (to allow for instrumentation not rated for full ocean depth such as a PAR sensor). One indirect objective will be to continue to collaboratively evaluate a BIOS-based glider program with Hydrostation S data as critically important ground truthing data. The existing glider program has challenging issue, and the aim of this effort is to use robust and highly accurate Hydrostation S data to test the capability of emerging technologies over the next five to ten years.

This project uses wide-baseline arrays of moored passive acoustic monitoring (PAM) recorders to record sounds produced by the critically endangered Rice’s whale ( Balaenoptera ricei ) in the northwestern Gulf of Mexico (GoMx). The data from this passive acoustic monitoring program will be used to increase knowledge of Rice’s whales’ spatiotemporal distribution and movement patterns in the GoMx to meet management needs. This project aims to support NMFS’s understanding, analyses, and management of human impacts on Rice’s whales in the GoMx.

Through Award 2305846 under Oceanographic Instrumentation, NSF has funded UAF to replace R/V Sikuliaq's existing EM302 multibeam echosounder (MBES), and upgrade to the Kongsberg EM304 Mark II MBES. As part of the installation, it is necessary to commission the new system by conducting Sea Acceptance Trials (SAT), before it can be considered operational. During the same dry docking period, UAF will also replace the failing receiver array on Sikuliaq's EM710 MBES, therefore a SAT is required for this modified system as well.

This cruise is part of an undergraduate immersion research course that will allow students to break away from the traditional class format and dive deeper into the world of microbial oceanography. Marine ecosystems are dominated by microscopic organisms that make up over two-thirds of ocean biomass and function as the major primary producers (phytoplankton) and consumers (bacteria). Our understanding of the marine microbial world has rapidly expanded with use of innovative molecular and chemical tools to uncover previously hidden taxonomic diversity, spatiotemporal distributions, and novel metabolic functions. Focusing on diversity and dynamics of phytoplankton and bacterial metabolisms, this interdisciplinary course provides a combination of laboratory, fieldwork, and data analyses to investigate marine microbes from the cellular to ecosystem scale. Undergraduate students will take part in a sampling expedition in coastal regions and use a variety of oceanographic equipment aboard a multi-day research cruise. Data types will span biogeochemistry (e.g., primary production, organic carbon, nutrients), marine microbe classification (e.g., microscopy, DNA sequencing), and metabolite identification (e.g., metabolomics). Follow-up wet bench cultivation techniques and data analysis will be carried out in the Durham Laboratory at UF.

Long-term observations over several decades are a powerful tool for investigating ocean physics, biology, and chemistry, and the response of the oceans to environmental change. The Bermuda Atlantic Time-Series Study, known as BATS, has been running continuously since 1988. The research goals of the BATS program are: (1) to improve our understanding of the time-varying components of the ocean carbon cycle and the cycles of related nutrient elements such as nitrogen, phosphorus, and silicon; and, (2) to identify the relevant physical, chemical and ecosystem properties responsible for this variability. In addition, the BATS program has strong and diverse broader impacts, contributing to the field of ocean sciences by providing high quality ocean observations and data for seagoing scientists and modelers, and a framework through which researchers can conceive and test hypotheses. This award will support the operations of the BATS program for five more years. The primary BATS research themes are as follows: (1) Quantify the role of ocean-atmosphere coupling and climate variability on air-sea exchange of CO2, and carbon export to the ocean interior; (2) Document trends and the controls on the interannual to decadal scale variability in carbon and nutrient cycles to their coupling in the surface and deep ocean via the Redfield Ratio paradigm; (3) Quantify the response of planktonic community structure and function, and impact on biogeochemical cycles to variability in surface fluxes and dynamical processes; (4) Facilitate development, calibration and validation of next generation oceanographic sensors, tools and technologies; and, (5) Generate a dataset that can be utilized by empiricists, modelers and students. This research integrates ocean physics, chemistry and biology into a framework for understanding oceanic processes and ocean change in the North Atlantic subtropical gyre. The existing 29 years of BATS data provide robust constraints on seasonal and interannual variability, the response of the Sargasso Sea ecosystem to natural climate variability, and signal detection of potential ocean changes. This project would extend the BATS program through years 31-35 to address a series of ten interlinked questions through integrated research approaches and a multitude of collaborative efforts. In addition to the themes above, and embedded into the ten questions and approaches, the BATS team will focus on, for example, coupling of particle production and biogeochemistry; revisiting the complexities of the biological carbon pump; oxygen decline; and changes in the hydrography, physics, ocean carbon cycle and biogeochemistry of the Sargasso Sea. The highest quality data observation and collection will be maintained and used to address these questions. Importantly, a wide range of collaborations at the BATS site, spanning the physical and biogeochemical disciplines, will aid these broad goals. Strong links to community stakeholders, and close collaboration (including methods intercomparisons and personnel exchanges) with the Hawaii Ocean Time-series are proposed. This work will extend the research findings of the project into educational and training opportunities within and beyond the oceanographic community, including training and mentorship of both undergraduate and graduate students.

One of the longest open-ocean hydrographic stations in the world is maintained at the Hydrostation S site (formerly known as the Panulirus site) located ~25 km southeast of Bermuda in the North Atlantic Ocean. This program of repeat biweekly hydrographic observations began in 1954 (by Hank Stommel) and now, in its seventh decade, has proved to be the catalyst for numerous studies of ocean physics, biological processes and biogeochemistry. Hydrostation S continues to be recognized as one of the most important sustained ocean time–series. The renewal of the Hydrostation S program is proposed herein, extending this invaluable time–series of ocean hydrography to almost 70 years. The upper ocean warming trend has strengthened (about +1.4° C since the 1970's; ~+0.8°C in last decade) with significant salinification (+0.16). Deeper waters such as the Labrador Sea Water has cooled by a few tenths of a degree and freshened with a marked decadal oscillation. The signature of deoxygenation has been observed at Hydrostation S in the upper ocean (about 4 µmoles kg -1 decade -1 decrease in dissolved oxygen) as well as the oxygen minimum zone (~2.5 µmoles kg -1 decade -1 ). Hydrostation S also supports the longest global ocean CO 2 and acidification time-series (from 1983 to present) The major objective of the proposal is to continue Hydrostation S into the eighth decade with numerous questions related to warming and cooling, salinification and freshening, deoxygenation and insights on biogeochemical changes over time. This program constitutes frequent water column sampling of temperature, salinity, and dissolved oxygen (and indirectly, sampling of important ocean carbon time–series) of the North Atlantic subtropical gyre at the Hydrostation S site. Such work is complementary to other sustained observations such as the Bermuda Atlantic Time–series Study (BATS) and Ocean Flux Program (OFP). We propose to carry out a similar sampling format that has been followed for the past 68 years. As for the past ten years, we would continue to conduct two CTD profiles to better capture the deep–water variability while maintaining all the previous discrete depths. The first CTD cast will profile to full ocean depth (3,300–3,500 m) while the second CTD cast will profile to 500 m (to allow for instrumentation not rated for full ocean depth such as a PAR sensor). One indirect objective will be to continue to collaboratively evaluate a BIOS-based glider program with Hydrostation S data as critically important ground truthing data. The existing glider program has challenging issue, and the aim of this effort is to use robust and highly accurate Hydrostation S data to test the capability of emerging technologies over the next five to ten years.

The most important and basic climate system components are the heat, freshwater, and carbon content and transport of the oceans, which directly interact with other essential components of the climate system in the atmosphere and cryosphere. In turn the associated variability and trends in the ocean's carbon content, carbonate saturation state, oxygen, and nutrients impact its internal biological environment, with notable large-scale regional changes. This joint study of the ocean carbon cycle and circulation is critical to identify critical areas where changes in ocean circulation could have serious consequences for future anthropogenic carbon uptake. Global warming-induced changes in the ocean's transport of heat and freshwater, which could affect the circulation, are being followed through these long-term measurements. Results to date have shown that the abyssal ocean is warming, taking up to 30% of the excess heat in the entire Earth system, leading to changes in circulation patterns and ventilation rates in the upper ocean. The uptake of anthropogenic carbon from the atmosphere to the ocean has been mapped and shows that the oceans are acidifying. Oxygen concentrations are declining in the ocean thermocline, and tropical oxygen minimum zones are expanding. The project is based on the fundamental concept that data collected belong to the community and need to be made quickly and freely available to the community at large through a recognized international data center. The project will continue to promote training and learning for graduate students, postdoctoral scientists, and new scientists in sea-going work. Outreach activities will continue as opportunities arise. The systematic and global re-occupation of select ocean hydrographic sections conducted for the past decade (2003-2014) will be extended into the 2015-2020 period with the continued objective of quantifying changes in the full-depth ocean. The project is in support of the U.S. CLIVAR (CLImate Variability and predictability) and the Carbon Science Programs, and is a component of a global observing system for the physical climate and carbon system. This long-standing project continues to contribute to the following overlapping scientific objectives: Data for Model Calibration, Validation and Model Based Synthesis; Carbon System Studies; Heat and Freshwater Storage and Flux Studies; Deep and Shallow Water Mass and Ventilation Studies; and Calibration of Autonomous Sensors. By integrating the scientific needs of the carbon, tracer and hydrographic communities, major scientific synergies and cost savings will continue to be achieved. In addition to efficiency, the coordinated approach produces scientific advances that exceed those of having individual programs. An average of two sections will be conducted every year, sometime collaboratively with international partner. The measurements made on each section include hydrography (full-depth vertical profiles of salinity, temperature, oxygen, nutrients, currents), Underway surface temperature, salinity, partial pressure of carbon dioxide (pCO2), air-sea fluxes, bathymetry, navigation), carbon system (dissolved inorganic carbon, pCO2. Total Alkalinity, pH, dissolved organic carbon and nitrogen), and transient tracers. This project will collect the data and perform the quality control measures routinely carried out by providers of reference-quality data. Post-cruise data updates, distribution, and archive will continue to be managed by groups with separate funding. This ongoing project is integrated with a larger international effort to monitor the ocean's response to climate change.

ALVIN engineering dives during McDermott cruise

Collaborative Research: Monitoring hydrothermal fluid origin, crustal permeability and seafloor morphology in preparation for the next volcanic eruption at the East Pacific Rise axis, 9° 45’-53’N NSF/OCE/MGG - 1949485 - Funded The East Pacific Rise (EPR) is a fast-spreading mid-ocean ridge located in the eastern Pacific Ocean. It is part of a globe-encircling system of ridges where the crust of the Earth is created by the intrusion of melt from the deeper mantle and consequent volcanic eruptions. Hydrothermal vents from the MOR form chimneys and columns of metal-rich emissions as seawater flows through the oceanic crust. These hydrothermal processes alter the composition of oceanic lithosphere and the global ocean, and provide unique habitats for extreme forms of life in chemosynthetic ecosystems. Understanding the coupling between sub-surface flow patterns and crustal conditions is fundamentally important for virtually all fields of mid-ocean ridge and related geochemical and geophysical research. This research project focuses on the idea that a new magmatic event involving an eruption or shallow magma emplacement, is likely to happen at the EPR axis in the 9°50?N area in the next few years (~2020?2023). This forecast is based on observed increases in vent exit-fluid temperature, and chemical trends. The scientists at sea will employ the research submersible Alvin and/or remotely operated vehicle (ROV) Jason to deploy instruments and collect vent fluid samples during three cruises over three successive years to study these processes. The project also involves a collaboration between young investigators from the USA and Norway that will help strengthen international ties between researchers in both countries. The researchers will also create new teaching materials for the San Diego Unified School district via three workshops held at Scripps Institution of Oceanography. This collaboration with the San Diego County Office of Education will enhance their Environmental Literacy Initiative, and help to create a Next Generation Science Standards,activities and resources for middle and/or high school teachers in California and nation-wide. This project's research objective focuses on the idea that a new magmatic event, either an eruption or shallow dike emplacement, is likely to happen at the EPR axis in the 9°50?N area in the next few years (~2020?2023). This forecast is based on increases in vent exit-fluid temperature and chemical trends measured at hydrothermal vents. The build-up to this event is anticipated to be associated with changes in crustal dynamics that can be observed in the geochemical properties and thermal response to tidal forcing across multiple hydrothermal vents and their fluids. Specifically, the phase lag and amplitude ratio between the thermal response and loading signal can be used to track the run-up to the next volcanic eruption. In order to monitor the relationship between magma resupply and its impact on hydrothermal fluid flow, the project will apply numerical methods to exploit the tidal variability observed in exit-fluid temperature records acquired using self-recording high-temperature loggers. The result of this analysis will constrain poroelastic properties of the crust. To accomplish these goals, the project will collect several correlative datasets on three successive cruises spaced approximately 1-year apart, with seven Alvin or Jason ROV dives each year. The datasets include i) vent exit-fluid temperature records logged at ~15 min intervals; ii) annual fluid samples from each instrumented vent using isobaric gas-tight samplers; iii) tidal pressure and current strength data logged by moored sensors; iv) high-resolution still and 4k-resolution video imagery of vent structures that are instrumented, and v) baseline near-bottom bathymetry and sidescan dataset to be acquired in December 2019 using autonomous underwater vehicle Sentry. These data will be a key resource to underpin the work of geophysicists, biologists and chemists from across the mid-ocean ridge community working at EPR 9°50?N. eruption.

The California Current Ecosystem (CCE) Long-Term Ecological Research (LTER) site is a coastal upwelling biome and the site of a 73-year (ongoing) spatially-resolved ocean timeseries (CalCOFI) that has demonstrated physical forcing on multiple spatiotemporal scales with concomitant impacts on ocean biota. The CCE is part of the larger LTER Network, founded in 1980 by the National Science Foundation. CCE-LTER studies are making excellent progress towards diagnosing mechanisms of ecosystem change in a major upwelling ecosystem and developing a quantitative framework for forecasting future changes. Our field site is in the CA Current upwelling regime, centered around Point Conception, within the CalCOFI time series station plan. The CCE LTER field program includes several different elements: experimental process cruises, time series cruises (ship time augmented on existing CalCOFI cruises) and mooring servicing cruises to maintain the deep water CCE1 and CCE2 moorings. The science carried out on these field expeditions is highly interdisciplinary, involving elements of biological, chemical, and physical oceanography.

Long-term observations over several decades are a powerful tool for investigating ocean physics, biology, and chemistry, and the response of the oceans to environmental change. The Bermuda Atlantic Time-Series Study, known as BATS, has been running continuously since 1988. The research goals of the BATS program are: (1) to improve our understanding of the time-varying components of the ocean carbon cycle and the cycles of related nutrient elements such as nitrogen, phosphorus, and silicon; and, (2) to identify the relevant physical, chemical and ecosystem properties responsible for this variability. In addition, the BATS program has strong and diverse broader impacts, contributing to the field of ocean sciences by providing high quality ocean observations and data for seagoing scientists and modelers, and a framework through which researchers can conceive and test hypotheses. This award will support the operations of the BATS program for five more years. The primary BATS research themes are as follows: (1) Quantify the role of ocean-atmosphere coupling and climate variability on air-sea exchange of CO2, and carbon export to the ocean interior; (2) Document trends and the controls on the interannual to decadal scale variability in carbon and nutrient cycles to their coupling in the surface and deep ocean via the Redfield Ratio paradigm; (3) Quantify the response of planktonic community structure and function, and impact on biogeochemical cycles to variability in surface fluxes and dynamical processes; (4) Facilitate development, calibration and validation of next generation oceanographic sensors, tools and technologies; and, (5) Generate a dataset that can be utilized by empiricists, modelers and students. This research integrates ocean physics, chemistry and biology into a framework for understanding oceanic processes and ocean change in the North Atlantic subtropical gyre. The existing 29 years of BATS data provide robust constraints on seasonal and interannual variability, the response of the Sargasso Sea ecosystem to natural climate variability, and signal detection of potential ocean changes. This project would extend the BATS program through years 31-35 to address a series of ten interlinked questions through integrated research approaches and a multitude of collaborative efforts. In addition to the themes above, and embedded into the ten questions and approaches, the BATS team will focus on, for example, coupling of particle production and biogeochemistry; revisiting the complexities of the biological carbon pump; oxygen decline; and changes in the hydrography, physics, ocean carbon cycle and biogeochemistry of the Sargasso Sea. The highest quality data observation and collection will be maintained and used to address these questions. Importantly, a wide range of collaborations at the BATS site, spanning the physical and biogeochemical disciplines, will aid these broad goals. Strong links to community stakeholders, and close collaboration (including methods intercomparisons and personnel exchanges) with the Hawaii Ocean Time-series are proposed. This work will extend the research findings of the project into educational and training opportunities within and beyond the oceanographic community, including training and mentorship of both undergraduate and graduate students.

One of the longest open-ocean hydrographic stations in the world is maintained at the Hydrostation S site (formerly known as the Panulirus site) located ~25 km southeast of Bermuda in the North Atlantic Ocean. This program of repeat biweekly hydrographic observations began in 1954 (by Hank Stommel) and now, in its seventh decade, has proved to be the catalyst for numerous studies of ocean physics, biological processes and biogeochemistry. Hydrostation S continues to be recognized as one of the most important sustained ocean time–series. The renewal of the Hydrostation S program is proposed herein, extending this invaluable time–series of ocean hydrography to almost 70 years. The upper ocean warming trend has strengthened (about +1.4° C since the 1970's; ~+0.8°C in last decade) with significant salinification (+0.16). Deeper waters such as the Labrador Sea Water has cooled by a few tenths of a degree and freshened with a marked decadal oscillation. The signature of deoxygenation has been observed at Hydrostation S in the upper ocean (about 4 µmoles kg -1 decade -1 decrease in dissolved oxygen) as well as the oxygen minimum zone (~2.5 µmoles kg -1 decade -1 ). Hydrostation S also supports the longest global ocean CO 2 and acidification time-series (from 1983 to present) The major objective of the proposal is to continue Hydrostation S into the eighth decade with numerous questions related to warming and cooling, salinification and freshening, deoxygenation and insights on biogeochemical changes over time. This program constitutes frequent water column sampling of temperature, salinity, and dissolved oxygen (and indirectly, sampling of important ocean carbon time–series) of the North Atlantic subtropical gyre at the Hydrostation S site. Such work is complementary to other sustained observations such as the Bermuda Atlantic Time–series Study (BATS) and Ocean Flux Program (OFP). We propose to carry out a similar sampling format that has been followed for the past 68 years. As for the past ten years, we would continue to conduct two CTD profiles to better capture the deep–water variability while maintaining all the previous discrete depths. The first CTD cast will profile to full ocean depth (3,300–3,500 m) while the second CTD cast will profile to 500 m (to allow for instrumentation not rated for full ocean depth such as a PAR sensor). One indirect objective will be to continue to collaboratively evaluate a BIOS-based glider program with Hydrostation S data as critically important ground truthing data. The existing glider program has challenging issue, and the aim of this effort is to use robust and highly accurate Hydrostation S data to test the capability of emerging technologies over the next five to ten years.

NES-LTER cross-shelf transect cruises are conducted between Martha’s Vineyard and the continental shelf break directly south. The overarching goal of the NES-LTER is to understand and predict how planktonic food webs change through space and time in response to changes in the physical environment, and how those changes impact ecosystem productivity, including of higher trophic levels. Observations and models are focused on processes and mechanisms that structure pelagic food webs, trophic transformations, and productivity, as well as the key interactions and feedbacks that modulate relationships among them. NES-LTER transect process cruises are focused on characterizing cross-shelf distributions of physical properties, nutrients, production rates, and plankton. Approximately 11 stations will be occupied in waters depths typically less than 200 m, but up to ~1600 m. Operations will include underway sampling of biological and chemical properties with science-provided instruments, CTD/rosette casts, net tows, on-deck incubations, and deployment of a towed vehicle.

The U.S. Global Ocean Ship-based Hydrographic Investigations Program (GO-SHIP) is part of the international GO-SHIP program , which carries out a systematic and global re-occupation of select hydrographic sections to quantify changes in storage and transport of heat, fresh water, carbon dioxide (CO2), and related parameters. By integrating the scientific needs of the carbon and hydrography/tracer communities, major synergies and cost savings are achieved. In addition to efficiency, the coordinated approach produces scientific advances that exceed those of individual programs. These advances contribute to the following overlapping scientific objectives: i) Data for Model Calibration and Validation; 2) Carbon System Studies; 3) Heat and Freshwater Storage and Flux Studies; 4) Deep and Shallow Water Mass and Ventilation Studies; and 5) Calibration of Autonomous Sensors. The most important and basic climate system components are the heat, freshwater, and carbon content and transport of the oceans, which directly interact with other essential components of the climate system in the atmosphere and cryosphere. In turn, the associated variability and trends in the ocean's carbon content, carbonate saturation state, oxygen, and nutrients impact its internal biological environment, with notable large-scale regional changes. This joint study of the ocean carbon cycle and circulation is critical to identify critical areas where changes in ocean circulation could have serious consequences for future anthropogenic carbon uptake. Global warming-induced changes in the ocean's transport of heat and freshwater, which could affect the circulation, are being followed through these long-term measurements. Results to date have shown that the abyssal ocean is warming, taking up to 30% of the excess heat in the entire Earth system, leading to changes in circulation patterns and ventilation rates in the upper ocean. The uptake of anthropogenic carbon from the atmosphere to the ocean has been mapped and shows that the oceans are acidifying. Oxygen concentrations are declining in the ocean thermocline, and tropical oxygen minimum zones are expanding. The project is based on the fundamental concept that data collected belong to the community and need to be made quickly and freely available to the community at large through a recognized international data center. The project will continue to promote training and learning for graduate students, postdoctoral scientists, and new scientists in sea-going work. Outreach activities will continue as opportunities arise. By integrating the scientific needs of the carbon, tracer and hydrographic communities, major scientific synergies and cost savings will continue to be achieved. In addition to efficiency, the coordinated approach produces scientific advances that exceed those of having individual programs. The measurements made on this section include hydrography (full-depth vertical profiles of salinity, temperature, oxygen, nutrients, currents), Underway surface temperature, salinity, partial pressure of carbon dioxide (pCO2), air-sea fluxes, bathymetry, navigation), carbon system (dissolved inorganic carbon, pCO2. Total Alkalinity, pH, dissolved organic carbon and nitrogen), and transient tracers. This project will collect the data and perform the quality control measures routinely carried out by providers of reference-quality data. Post-cruise data updates, distribution, and archive will continue to be managed by groups with separate funding. This ongoing project is integrated with a larger international effort to monitor the ocean's response to climate change. The focus of this particular cruise is to determine the changes in anthropogenic CO2, distributions and fluxes in the South Atlantic since the last occupation in 2010 as part of the GO-SHIP program. Decadal variations of CO2 tracer, oxygen, and temperature distributions are strongly influenced by climate change and natural processes. The GO-SHIP cruises are the only means to obtain climate quality data to study changes and impacts in the ocean. This research is co-sponsored by NOAA and NSF.

One of the longest open-ocean hydrographic stations in the world is maintained at the Hydrostation S site (formerly known as the Panulirus site) located ~25 km southeast of Bermuda in the North Atlantic Ocean. This program of repeat biweekly hydrographic observations began in 1954 (by Hank Stommel) and now, in its seventh decade, has proved to be the catalyst for numerous studies of ocean physics, biological processes and biogeochemistry. Hydrostation S continues to be recognized as one of the most important sustained ocean time–series. The renewal of the Hydrostation S program is proposed herein, extending this invaluable time–series of ocean hydrography to almost 70 years. The upper ocean warming trend has strengthened (about +1.4° C since the 1970's; ~+0.8°C in last decade) with significant salinification (+0.16). Deeper waters such as the Labrador Sea Water has cooled by a few tenths of a degree and freshened with a marked decadal oscillation. The signature of deoxygenation has been observed at Hydrostation S in the upper ocean (about 4 µmoles kg -1 decade -1 decrease in dissolved oxygen) as well as the oxygen minimum zone (~2.5 µmoles kg -1 decade -1 ). Hydrostation S also supports the longest global ocean CO 2 and acidification time-series (from 1983 to present) The major objective of the proposal is to continue Hydrostation S into the eighth decade with numerous questions related to warming and cooling, salinification and freshening, deoxygenation and insights on biogeochemical changes over time. This program constitutes frequent water column sampling of temperature, salinity, and dissolved oxygen (and indirectly, sampling of important ocean carbon time–series) of the North Atlantic subtropical gyre at the Hydrostation S site. Such work is complementary to other sustained observations such as the Bermuda Atlantic Time–series Study (BATS) and Ocean Flux Program (OFP). We propose to carry out a similar sampling format that has been followed for the past 68 years. As for the past ten years, we would continue to conduct two CTD profiles to better capture the deep–water variability while maintaining all the previous discrete depths. The first CTD cast will profile to full ocean depth (3,300–3,500 m) while the second CTD cast will profile to 500 m (to allow for instrumentation not rated for full ocean depth such as a PAR sensor). One indirect objective will be to continue to collaboratively evaluate a BIOS-based glider program with Hydrostation S data as critically important ground truthing data. The existing glider program has challenging issue, and the aim of this effort is to use robust and highly accurate Hydrostation S data to test the capability of emerging technologies over the next five to ten years.

Engineering Testing of Acoustic, Imaging, and Microstructure profilers.

In recent years, oil and gas facilities have aggressively extended into the ultra-deep waters (>1,000 m) in the Gulf of Mexico (GoM), impinging upon the offshore habitats of both large epipelagic predators and diel vertically migrating (DVM) mesopelagics. High intensity lighting at these platforms attracts a variety of prey at night including lanternfishes (Myctophidae) and other vertically migrating fishes, squids and zooplankton. As well, a number of pelagic apex predators including yellowfin tuna Thunnus albacares , blackfin tuna Thunnus atlanticus , and other species congregate, presumably to feed and seek protection near these platforms, and lucrative recreational fisheries there result. A pilot study we conducted in 2020 near four of these platforms confirmed the preponderance of mesopelagics in predator stomachs, contrasting sharply with previous food habits studies of tunas widely distributed in epipelagic waters. The goals of our project are to: (1) better understand the effects that large, lighted oil production facilities have on vertically migrating animals and how the presence of such facilities may impact trade-offs between greater growth potential in surface waters vs. greater predation risks, and (2) determine if the presence of such facilities acts to congregate large pelagic species such as tunas, and especially small/young fish, thus serving as fish aggregating devices (FADs).

Long-term observations over several decades are a powerful tool for investigating ocean physics, biology, and chemistry, and the response of the oceans to environmental change. The Bermuda Atlantic Time-Series Study, known as BATS, has been running continuously since 1988. The research goals of the BATS program are: (1) to improve our understanding of the time-varying components of the ocean carbon cycle and the cycles of related nutrient elements such as nitrogen, phosphorus, and silicon; and, (2) to identify the relevant physical, chemical and ecosystem properties responsible for this variability. In addition, the BATS program has strong and diverse broader impacts, contributing to the field of ocean sciences by providing high quality ocean observations and data for seagoing scientists and modelers, and a framework through which researchers can conceive and test hypotheses. This award will support the operations of the BATS program for five more years. The primary BATS research themes are as follows: (1) Quantify the role of ocean-atmosphere coupling and climate variability on air-sea exchange of CO2, and carbon export to the ocean interior; (2) Document trends and the controls on the interannual to decadal scale variability in carbon and nutrient cycles to their coupling in the surface and deep ocean via the Redfield Ratio paradigm; (3) Quantify the response of planktonic community structure and function, and impact on biogeochemical cycles to variability in surface fluxes and dynamical processes; (4) Facilitate development, calibration and validation of next generation oceanographic sensors, tools and technologies; and, (5) Generate a dataset that can be utilized by empiricists, modelers and students. This research integrates ocean physics, chemistry and biology into a framework for understanding oceanic processes and ocean change in the North Atlantic subtropical gyre. The existing 29 years of BATS data provide robust constraints on seasonal and interannual variability, the response of the Sargasso Sea ecosystem to natural climate variability, and signal detection of potential ocean changes. This project would extend the BATS program through years 31-35 to address a series of ten interlinked questions through integrated research approaches and a multitude of collaborative efforts. In addition to the themes above, and embedded into the ten questions and approaches, the BATS team will focus on, for example, coupling of particle production and biogeochemistry; revisiting the complexities of the biological carbon pump; oxygen decline; and changes in the hydrography, physics, ocean carbon cycle and biogeochemistry of the Sargasso Sea. The highest quality data observation and collection will be maintained and used to address these questions. Importantly, a wide range of collaborations at the BATS site, spanning the physical and biogeochemical disciplines, will aid these broad goals. Strong links to community stakeholders, and close collaboration (including methods intercomparisons and personnel exchanges) with the Hawaii Ocean Time-series are proposed. This work will extend the research findings of the project into educational and training opportunities within and beyond the oceanographic community, including training and mentorship of both undergraduate and graduate students.

One of the longest open-ocean hydrographic stations in the world is maintained at the Hydrostation S site (formerly known as the Panulirus site) located ~25 km southeast of Bermuda in the North Atlantic Ocean. This program of repeat biweekly hydrographic observations began in 1954 (by Hank Stommel) and now, in its seventh decade, has proved to be the catalyst for numerous studies of ocean physics, biological processes and biogeochemistry. Hydrostation S continues to be recognized as one of the most important sustained ocean time–series. The renewal of the Hydrostation S program is proposed herein, extending this invaluable time–series of ocean hydrography to almost 70 years. The upper ocean warming trend has strengthened (about +1.4° C since the 1970's; ~+0.8°C in last decade) with significant salinification (+0.16). Deeper waters such as the Labrador Sea Water has cooled by a few tenths of a degree and freshened with a marked decadal oscillation. The signature of deoxygenation has been observed at Hydrostation S in the upper ocean (about 4 µmoles kg -1 decade -1 decrease in dissolved oxygen) as well as the oxygen minimum zone (~2.5 µmoles kg -1 decade -1 ). Hydrostation S also supports the longest global ocean CO 2 and acidification time-series (from 1983 to present) The major objective of the proposal is to continue Hydrostation S into the eighth decade with numerous questions related to warming and cooling, salinification and freshening, deoxygenation and insights on biogeochemical changes over time. This program constitutes frequent water column sampling of temperature, salinity, and dissolved oxygen (and indirectly, sampling of important ocean carbon time–series) of the North Atlantic subtropical gyre at the Hydrostation S site. Such work is complementary to other sustained observations such as the Bermuda Atlantic Time–series Study (BATS) and Ocean Flux Program (OFP). We propose to carry out a similar sampling format that has been followed for the past 68 years. As for the past ten years, we would continue to conduct two CTD profiles to better capture the deep–water variability while maintaining all the previous discrete depths. The first CTD cast will profile to full ocean depth (3,300–3,500 m) while the second CTD cast will profile to 500 m (to allow for instrumentation not rated for full ocean depth such as a PAR sensor). One indirect objective will be to continue to collaboratively evaluate a BIOS-based glider program with Hydrostation S data as critically important ground truthing data. The existing glider program has challenging issue, and the aim of this effort is to use robust and highly accurate Hydrostation S data to test the capability of emerging technologies over the next five to ten years.

ALVIN engineering dive during Arellano cruise

Hydrothermal venting occurs globally on the deep seafloor and has been the subject of vigorous geological, chemical and biological research. This venting, however, is ultimately transient, leaving sulfide mineral-rich deposits behind after the fluid flow stops. We propose to investigate the organisms living on these inactive sulfide features in order to understand their feeding ecology and associations with the mineral substratum. Recent discoveries indicate that some microbial and animal species inhabiting inactive sulfides are not found elsewhere in the marine environment. Our main objectives are to characterize the species and functional diversity of these communities (eukaryotic, bacterial and archaeal), determine which species are endemic or predominantly associated with inactive sulfides, and explore the processes underlying those obligate associations. Specifically, we will determine whether a unique trophic web exists on these features, consisting of rock-hosted chemolithotrophic microorganisms consumed by small grazers, and investigate the associations between minerals, microorganisms and animals over a broad range of mineral characteristics. We will conduct field studies near the active vent fields between 9-10°N on the East Pacific Rise, using camera surveys and sampling organisms on the rocks and in the overlying water column overlying water column, followed by laboratory studies of petrology, molecular genetics, phylogenetics and feeding ecology.

Over four decades of research have shown that tiny free-swimming offspring of the unique inhabitants of hydrothermal vents can disperse effectively between their specialized habitats. Yet, we know almost nothing about how these larval animals complete the journey by locating and settling down in suitable locations. This question remains one of the key unresolved puzzles in the ecology of the deep sea and is becoming increasingly important to solve as hydrothermal vents are becoming threatened by human impacts. The investigators suggest that the films of bacteria that first form at vents are good signposts for settlement of larvae because they indicate that the hydrothermal vents are suitable for life. This project uses a combined program of field experiments, cutting-edge molecular biology techniques, and shipboard experiments with hydrothermal-vent larvae and cultured bacterial films. The project also connects undergraduate research interns at a primarily undergraduate institution (Western Washington University) with undergraduate research interns at two research institutions (Rutgers and Woods Hole Oceanographic Institution) while working on the project at sea together. Finally, the team is producing a science-in-action documentary filled with ocean science and exploration intended for television distribution and museum screenings. The investigators are using footage of the deep-sea vents, shipboard and diving operations, and laboratory work to create a documentary that highlights the foundation of scientific research?hypothesis-driven research, the application of the scientific method, and the importance of critical thinking?all in the framework of the study of an exciting, but threatened, ecosystem. Hydrothermal vents are particularly tractable systems in which to study questions about the roles of biofilms in larval settlement because biofilms at vents are relatively low-complexity; vent animals are strictly dependent on vent microbes, often through symbiotic partnerships acquired after settlement; and environmental variations are present within the range of a common larval pool. Moreover, decades of research on settlement in model organisms give us good insight into biofilm cues; there is solid foundational understanding about colonization patterns at vents; we now have excellent tools to collect, identify, and culture vent larvae and microbes; and modern environmental "-omics" techniques are a good tool to characterize biological cues produced by biofilms. The project provides an unprecedented, quantitative look into the role of microbial biofilms in structuring larval settlement at hydrothermal vents, achieved only through the close collaboration of microbial and larval ecologists. The combined field program of short-term settlement experiments, microbial "-omics" work, and subsequent shipboard settlement experiments allows the investigative team to use field experiments to statistically model the factors that best predict larval settlement in the field, then test those predictions with shipboard experiments that decouple covarying conditions. This extensive characterization of putative larval settlement cues and their relationship to colonization success in heterogeneous vent habitat niches will contribute to a broader understanding of colonization success across diverse marine ecosystems. Understanding the role that the initial settlement of larvae plays in the recovery and resilience of hydrothermal-vent ecosystems is critical to developing informed management plans for deep-sea mining.